Protective effect of zuogui pill on ovarian autoimmune injury

CONCLUSION: ZGP (Chinese herbal formula) can improve immune inflammatory injury of ovary, and shows therapeutic effect on POF.

Zhu L, Luo SP, Xu LM. Department of TCM, Southern Medical University, Guangzhou. zhuling20012001 [at] yahoo [dot] com

OBJECTIVE:
To probe the effect of Zuogui pill (ZGP), a Chinese compound recipe for
tonifying Shen, on ovarian function in mice with premature ovarian
failure (POF). METHODS: BALB/C female mice model of POF was established
by multiple sites subcutaneous injection of ovarian antigen elicited
with ovarian tissue of SD female rats, and treated with ZGP at
different time points in the modeling, with prednisone as positive
control. The levels of follicle-stimulating hormone (FSH) and estradiol
(E2) in peripheral blood were measured with radioimmunoassay, and
ovarian antibody (AoAb) was determined by enzyme linked immunosorbent
assay. The mRNA expression of ovarian growth and differentiation
factor-9 was detected with in situ hybridization. RESULTS: POF model
mice manifested such abnormalities as increased FSH, decreased E2, and
positive AoAb in peripheral blood, with lymphocytes infiltration in
ovarian mesanchyma, reduction of GDF-9 mRNA positive oocytes, and
decrease of growing and mature follicles. ZGP could reduce the increase
of FSH, increase the level of E2, inhibit the production of AoAb, raise
the GDF-9 mRNA positive cells of oocytes, increase the number of
growing and mature follicles. The clinical efficacy was more
significant in early stage than in advanced stage. CONCLUSION: ZGP can
improve immune inflammatory injury of ovary, and shows therapeutic
effect on POF.