Preventive and therapeutic effects of Bushen Huoxue Recipe on autoimmune premature ovarian failure in mice

Conclusion: BSHXR (Chinese herbal formula) can recover ovarian function in POF mice mainly by regulating the indiscriminate hormone level, and BSHXR has preventive effect on autoimmune POF in mice.

Dong L, Jiang L, Men W, Zhu NS.    http://www.ncbi.nlm.nih.gov/pubmed/18334152

Department
of Gynecology, Yueyang Hospital of Integrated Traditional Chinese and
Western Medicine, Shanghai University of Traditional Chinese Medicine,
Shanghai 200437, China; E-mail: dongli888 [at] yahoo [dot] com.

Objective:
To investigate the preventive effect of Bushen Huoxue Recipe (BSHXR), a
compound traditional Chinese herbal medicine, on autoimmune premature
ovarian failure (POF) in mice. Methods: Ovarian antigen was prepared
with the ovarian tissue of female BALB/c mice. A mouse model of POF was
established by immunization injection of the ovarian antigen of isotype
female mice on multiple subcutaneous sites and two posterior soles. The
POF mice were treated with BSHXR after the first and third
immunization. The levels of follicle-stimulating hormone (FSH),
luteinizing hormone (LH) and estradiol (E2) in peripheral blood were
detected by enzyme linked immunosorbent assay. Results: Lymphocyte
infiltration was appeared in ovarian stroma of POF mice. The levels of
FSH and LH were evaluated and the E(2) level was decreased
significantly (P<0.05). BSHXR could reduce the increased levels of
FSH and LH, increase the level of E(2) and the number of growing and
mature follicles. The efficacy of early treatment was better than that
of late treatment. Conclusion: BSHXR can recover ovarian function in
POF mice mainly by regulating the indiscriminate hormone level, and
BSHXR has preventive effect on autoimmune POF in mice.

PMID: 18334152 [PubMed - in process]